Review



placzi gw reporter vectors  (TaKaRa)


Bioz Verified Symbol TaKaRa is a verified supplier
Bioz Manufacturer Symbol TaKaRa manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 86
      Buy from Supplier

    Structured Review

    TaKaRa placzi gw reporter vectors
    (A) SPL9 interacts with <t>the</t> <t>MAC3A</t> and MAC3B promoters in the yeast one-hybrid assays. The yeast bearing the <t>pLacZi</t> promoter-reporter vectors and pGADT7 vectors expressing SPL9 were grown on synthetic dropout medium (SD-Leu-Ura-Met). The colonies were blotted on the filter paper to test the β-Galactosidase (β-Gal) activity. The blue color indicates the interaction between 1.5 kb promoter fragments of MAC3A and MAC3B in the pLacZi vectors and the transcription factors in the pGADT7 vectors. Empty vectors (e.v.) were used as negative control. (B) The chromatin immunoprecipitation (ChIP) assays in the Arabidopsis seeding expressing rSPL9-GFP or GFP only. The locations of SPL9 predicted binding sites (SPB-box, black boxes) were illustrated in the MAC3A and MAC3B promoters (upper panel). The amplicons were designed to surround SBP-Box (greys boxes labeled with A to H) or ACTIN2 ( ACT2 ) as controls. The chromatins in the Arabidopsis seedlings expressing rSPL9-GFP (dark blue bars) or GFP (grey bars) along with Col-0 were immunoprecipitated with anti-GFP antibody, and the relative enrichments of immunoprecipitated DNA relative to input were calculated as “%input.” shown as a bar graph. The fold enrichment (black dots) was calculated by dividing “%input” in SPL9-GFP with “%input” in GFP. The grey horizontal dashed line represents the fold enrichment of negative control, ACT2 . The “ND” stands for not detectable, and the transcription start site (TSS) and the translation initiation site (TIS) were labeled. Only one replicate is presented here.
    Placzi Gw Reporter Vectors, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/placzi+reporter+vector/bio_rxiv__2024__03__26__586198-272-16-35?v=TaKaRa
    Average 86 stars, based on 1 article reviews
    placzi gw reporter vectors - by Bioz Stars, 2026-08
    86/100 stars

    Images

    1) Product Images from "MOS4-Associated Complex subunits 3A and 3B modulate FLM splicing to repress photoperiod-dependent floral transition"

    Article Title: MOS4-Associated Complex subunits 3A and 3B modulate FLM splicing to repress photoperiod-dependent floral transition

    Journal: bioRxiv

    doi: 10.1101/2024.03.26.586198

    (A) SPL9 interacts with the MAC3A and MAC3B promoters in the yeast one-hybrid assays. The yeast bearing the pLacZi promoter-reporter vectors and pGADT7 vectors expressing SPL9 were grown on synthetic dropout medium (SD-Leu-Ura-Met). The colonies were blotted on the filter paper to test the β-Galactosidase (β-Gal) activity. The blue color indicates the interaction between 1.5 kb promoter fragments of MAC3A and MAC3B in the pLacZi vectors and the transcription factors in the pGADT7 vectors. Empty vectors (e.v.) were used as negative control. (B) The chromatin immunoprecipitation (ChIP) assays in the Arabidopsis seeding expressing rSPL9-GFP or GFP only. The locations of SPL9 predicted binding sites (SPB-box, black boxes) were illustrated in the MAC3A and MAC3B promoters (upper panel). The amplicons were designed to surround SBP-Box (greys boxes labeled with A to H) or ACTIN2 ( ACT2 ) as controls. The chromatins in the Arabidopsis seedlings expressing rSPL9-GFP (dark blue bars) or GFP (grey bars) along with Col-0 were immunoprecipitated with anti-GFP antibody, and the relative enrichments of immunoprecipitated DNA relative to input were calculated as “%input.” shown as a bar graph. The fold enrichment (black dots) was calculated by dividing “%input” in SPL9-GFP with “%input” in GFP. The grey horizontal dashed line represents the fold enrichment of negative control, ACT2 . The “ND” stands for not detectable, and the transcription start site (TSS) and the translation initiation site (TIS) were labeled. Only one replicate is presented here.
    Figure Legend Snippet: (A) SPL9 interacts with the MAC3A and MAC3B promoters in the yeast one-hybrid assays. The yeast bearing the pLacZi promoter-reporter vectors and pGADT7 vectors expressing SPL9 were grown on synthetic dropout medium (SD-Leu-Ura-Met). The colonies were blotted on the filter paper to test the β-Galactosidase (β-Gal) activity. The blue color indicates the interaction between 1.5 kb promoter fragments of MAC3A and MAC3B in the pLacZi vectors and the transcription factors in the pGADT7 vectors. Empty vectors (e.v.) were used as negative control. (B) The chromatin immunoprecipitation (ChIP) assays in the Arabidopsis seeding expressing rSPL9-GFP or GFP only. The locations of SPL9 predicted binding sites (SPB-box, black boxes) were illustrated in the MAC3A and MAC3B promoters (upper panel). The amplicons were designed to surround SBP-Box (greys boxes labeled with A to H) or ACTIN2 ( ACT2 ) as controls. The chromatins in the Arabidopsis seedlings expressing rSPL9-GFP (dark blue bars) or GFP (grey bars) along with Col-0 were immunoprecipitated with anti-GFP antibody, and the relative enrichments of immunoprecipitated DNA relative to input were calculated as “%input.” shown as a bar graph. The fold enrichment (black dots) was calculated by dividing “%input” in SPL9-GFP with “%input” in GFP. The grey horizontal dashed line represents the fold enrichment of negative control, ACT2 . The “ND” stands for not detectable, and the transcription start site (TSS) and the translation initiation site (TIS) were labeled. Only one replicate is presented here.

    Techniques Used: Expressing, Activity Assay, Negative Control, Chromatin Immunoprecipitation, Binding Assay, Labeling, Immunoprecipitation



    Similar Products

    placzi gw reporter vectors  (TaKaRa)
    86
    TaKaRa placzi gw reporter vectors
    (A) SPL9 interacts with <t>the</t> <t>MAC3A</t> and MAC3B promoters in the yeast one-hybrid assays. The yeast bearing the <t>pLacZi</t> promoter-reporter vectors and pGADT7 vectors expressing SPL9 were grown on synthetic dropout medium (SD-Leu-Ura-Met). The colonies were blotted on the filter paper to test the β-Galactosidase (β-Gal) activity. The blue color indicates the interaction between 1.5 kb promoter fragments of MAC3A and MAC3B in the pLacZi vectors and the transcription factors in the pGADT7 vectors. Empty vectors (e.v.) were used as negative control. (B) The chromatin immunoprecipitation (ChIP) assays in the Arabidopsis seeding expressing rSPL9-GFP or GFP only. The locations of SPL9 predicted binding sites (SPB-box, black boxes) were illustrated in the MAC3A and MAC3B promoters (upper panel). The amplicons were designed to surround SBP-Box (greys boxes labeled with A to H) or ACTIN2 ( ACT2 ) as controls. The chromatins in the Arabidopsis seedlings expressing rSPL9-GFP (dark blue bars) or GFP (grey bars) along with Col-0 were immunoprecipitated with anti-GFP antibody, and the relative enrichments of immunoprecipitated DNA relative to input were calculated as “%input.” shown as a bar graph. The fold enrichment (black dots) was calculated by dividing “%input” in SPL9-GFP with “%input” in GFP. The grey horizontal dashed line represents the fold enrichment of negative control, ACT2 . The “ND” stands for not detectable, and the transcription start site (TSS) and the translation initiation site (TIS) were labeled. Only one replicate is presented here.
    Placzi Gw Reporter Vectors, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/placzi+reporter+vector/bio_rxiv__2024__03__26__586198-272-16-35?v=TaKaRa
    Average 86 stars, based on 1 article reviews
    placzi gw reporter vectors - by Bioz Stars, 2026-08
    86/100 stars
    		  Buy from Supplier
    placzi reporter vector  (TaKaRa)
    94
    TaKaRa placzi reporter vector
    (A) SPL9 interacts with <t>the</t> <t>MAC3A</t> and MAC3B promoters in the yeast one-hybrid assays. The yeast bearing the <t>pLacZi</t> promoter-reporter vectors and pGADT7 vectors expressing SPL9 were grown on synthetic dropout medium (SD-Leu-Ura-Met). The colonies were blotted on the filter paper to test the β-Galactosidase (β-Gal) activity. The blue color indicates the interaction between 1.5 kb promoter fragments of MAC3A and MAC3B in the pLacZi vectors and the transcription factors in the pGADT7 vectors. Empty vectors (e.v.) were used as negative control. (B) The chromatin immunoprecipitation (ChIP) assays in the Arabidopsis seeding expressing rSPL9-GFP or GFP only. The locations of SPL9 predicted binding sites (SPB-box, black boxes) were illustrated in the MAC3A and MAC3B promoters (upper panel). The amplicons were designed to surround SBP-Box (greys boxes labeled with A to H) or ACTIN2 ( ACT2 ) as controls. The chromatins in the Arabidopsis seedlings expressing rSPL9-GFP (dark blue bars) or GFP (grey bars) along with Col-0 were immunoprecipitated with anti-GFP antibody, and the relative enrichments of immunoprecipitated DNA relative to input were calculated as “%input.” shown as a bar graph. The fold enrichment (black dots) was calculated by dividing “%input” in SPL9-GFP with “%input” in GFP. The grey horizontal dashed line represents the fold enrichment of negative control, ACT2 . The “ND” stands for not detectable, and the transcription start site (TSS) and the translation initiation site (TIS) were labeled. Only one replicate is presented here.
    Placzi Reporter Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/placzi+reporter+vector/pm37938892-299-8-12?v=TaKaRa
    Average 94 stars, based on 1 article reviews
    placzi reporter vector - by Bioz Stars, 2026-08
    94/100 stars
    		  Buy from Supplier
    reporter vector placzi pro  (TaKaRa)
    96
    TaKaRa reporter vector placzi pro
    (A) SPL9 interacts with <t>the</t> <t>MAC3A</t> and MAC3B promoters in the yeast one-hybrid assays. The yeast bearing the <t>pLacZi</t> promoter-reporter vectors and pGADT7 vectors expressing SPL9 were grown on synthetic dropout medium (SD-Leu-Ura-Met). The colonies were blotted on the filter paper to test the β-Galactosidase (β-Gal) activity. The blue color indicates the interaction between 1.5 kb promoter fragments of MAC3A and MAC3B in the pLacZi vectors and the transcription factors in the pGADT7 vectors. Empty vectors (e.v.) were used as negative control. (B) The chromatin immunoprecipitation (ChIP) assays in the Arabidopsis seeding expressing rSPL9-GFP or GFP only. The locations of SPL9 predicted binding sites (SPB-box, black boxes) were illustrated in the MAC3A and MAC3B promoters (upper panel). The amplicons were designed to surround SBP-Box (greys boxes labeled with A to H) or ACTIN2 ( ACT2 ) as controls. The chromatins in the Arabidopsis seedlings expressing rSPL9-GFP (dark blue bars) or GFP (grey bars) along with Col-0 were immunoprecipitated with anti-GFP antibody, and the relative enrichments of immunoprecipitated DNA relative to input were calculated as “%input.” shown as a bar graph. The fold enrichment (black dots) was calculated by dividing “%input” in SPL9-GFP with “%input” in GFP. The grey horizontal dashed line represents the fold enrichment of negative control, ACT2 . The “ND” stands for not detectable, and the transcription start site (TSS) and the translation initiation site (TIS) were labeled. Only one replicate is presented here.
    Reporter Vector Placzi Pro, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/placzi+reporter+vector/pmc08122443-163-2-27?v=TaKaRa
    Average 96 stars, based on 1 article reviews
    reporter vector placzi pro - by Bioz Stars, 2026-08
    96/100 stars
    		  Buy from Supplier
    placzi bait vectors containing a lacz reporter gene  (Thermo Fisher)
    90
    Thermo Fisher placzi bait vectors containing a lacz reporter gene
    (A) SPL9 interacts with <t>the</t> <t>MAC3A</t> and MAC3B promoters in the yeast one-hybrid assays. The yeast bearing the <t>pLacZi</t> promoter-reporter vectors and pGADT7 vectors expressing SPL9 were grown on synthetic dropout medium (SD-Leu-Ura-Met). The colonies were blotted on the filter paper to test the β-Galactosidase (β-Gal) activity. The blue color indicates the interaction between 1.5 kb promoter fragments of MAC3A and MAC3B in the pLacZi vectors and the transcription factors in the pGADT7 vectors. Empty vectors (e.v.) were used as negative control. (B) The chromatin immunoprecipitation (ChIP) assays in the Arabidopsis seeding expressing rSPL9-GFP or GFP only. The locations of SPL9 predicted binding sites (SPB-box, black boxes) were illustrated in the MAC3A and MAC3B promoters (upper panel). The amplicons were designed to surround SBP-Box (greys boxes labeled with A to H) or ACTIN2 ( ACT2 ) as controls. The chromatins in the Arabidopsis seedlings expressing rSPL9-GFP (dark blue bars) or GFP (grey bars) along with Col-0 were immunoprecipitated with anti-GFP antibody, and the relative enrichments of immunoprecipitated DNA relative to input were calculated as “%input.” shown as a bar graph. The fold enrichment (black dots) was calculated by dividing “%input” in SPL9-GFP with “%input” in GFP. The grey horizontal dashed line represents the fold enrichment of negative control, ACT2 . The “ND” stands for not detectable, and the transcription start site (TSS) and the translation initiation site (TIS) were labeled. Only one replicate is presented here.
    Placzi Bait Vectors Containing A Lacz Reporter Gene, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/placzi+reporter+vector/pmc04122305-186-19-37?v=Thermo+Fisher
    Average 90 stars, based on 1 article reviews
    placzi bait vectors containing a lacz reporter gene - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier
    placzi bait vectors containing lacz reporter gene  (Thermo Fisher)
    90
    Thermo Fisher placzi bait vectors containing lacz reporter gene
    (A) SPL9 interacts with <t>the</t> <t>MAC3A</t> and MAC3B promoters in the yeast one-hybrid assays. The yeast bearing the <t>pLacZi</t> promoter-reporter vectors and pGADT7 vectors expressing SPL9 were grown on synthetic dropout medium (SD-Leu-Ura-Met). The colonies were blotted on the filter paper to test the β-Galactosidase (β-Gal) activity. The blue color indicates the interaction between 1.5 kb promoter fragments of MAC3A and MAC3B in the pLacZi vectors and the transcription factors in the pGADT7 vectors. Empty vectors (e.v.) were used as negative control. (B) The chromatin immunoprecipitation (ChIP) assays in the Arabidopsis seeding expressing rSPL9-GFP or GFP only. The locations of SPL9 predicted binding sites (SPB-box, black boxes) were illustrated in the MAC3A and MAC3B promoters (upper panel). The amplicons were designed to surround SBP-Box (greys boxes labeled with A to H) or ACTIN2 ( ACT2 ) as controls. The chromatins in the Arabidopsis seedlings expressing rSPL9-GFP (dark blue bars) or GFP (grey bars) along with Col-0 were immunoprecipitated with anti-GFP antibody, and the relative enrichments of immunoprecipitated DNA relative to input were calculated as “%input.” shown as a bar graph. The fold enrichment (black dots) was calculated by dividing “%input” in SPL9-GFP with “%input” in GFP. The grey horizontal dashed line represents the fold enrichment of negative control, ACT2 . The “ND” stands for not detectable, and the transcription start site (TSS) and the translation initiation site (TIS) were labeled. Only one replicate is presented here.
    Placzi Bait Vectors Containing Lacz Reporter Gene, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/placzi+reporter+vector/10__1126_slash_scisignal__6277er3-157-18-35?v=Thermo+Fisher
    Average 90 stars, based on 1 article reviews
    placzi bait vectors containing lacz reporter gene - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier
    reporter vector placzi  (TaKaRa)
    96
    TaKaRa reporter vector placzi
    (A) SPL9 interacts with <t>the</t> <t>MAC3A</t> and MAC3B promoters in the yeast one-hybrid assays. The yeast bearing the <t>pLacZi</t> promoter-reporter vectors and pGADT7 vectors expressing SPL9 were grown on synthetic dropout medium (SD-Leu-Ura-Met). The colonies were blotted on the filter paper to test the β-Galactosidase (β-Gal) activity. The blue color indicates the interaction between 1.5 kb promoter fragments of MAC3A and MAC3B in the pLacZi vectors and the transcription factors in the pGADT7 vectors. Empty vectors (e.v.) were used as negative control. (B) The chromatin immunoprecipitation (ChIP) assays in the Arabidopsis seeding expressing rSPL9-GFP or GFP only. The locations of SPL9 predicted binding sites (SPB-box, black boxes) were illustrated in the MAC3A and MAC3B promoters (upper panel). The amplicons were designed to surround SBP-Box (greys boxes labeled with A to H) or ACTIN2 ( ACT2 ) as controls. The chromatins in the Arabidopsis seedlings expressing rSPL9-GFP (dark blue bars) or GFP (grey bars) along with Col-0 were immunoprecipitated with anti-GFP antibody, and the relative enrichments of immunoprecipitated DNA relative to input were calculated as “%input.” shown as a bar graph. The fold enrichment (black dots) was calculated by dividing “%input” in SPL9-GFP with “%input” in GFP. The grey horizontal dashed line represents the fold enrichment of negative control, ACT2 . The “ND” stands for not detectable, and the transcription start site (TSS) and the translation initiation site (TIS) were labeled. Only one replicate is presented here.
    Reporter Vector Placzi, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/placzi+reporter+vector/pmc02613660-292-7-10?v=TaKaRa
    Average 96 stars, based on 1 article reviews
    reporter vector placzi - by Bioz Stars, 2026-08
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) SPL9 interacts with the MAC3A and MAC3B promoters in the yeast one-hybrid assays. The yeast bearing the pLacZi promoter-reporter vectors and pGADT7 vectors expressing SPL9 were grown on synthetic dropout medium (SD-Leu-Ura-Met). The colonies were blotted on the filter paper to test the β-Galactosidase (β-Gal) activity. The blue color indicates the interaction between 1.5 kb promoter fragments of MAC3A and MAC3B in the pLacZi vectors and the transcription factors in the pGADT7 vectors. Empty vectors (e.v.) were used as negative control. (B) The chromatin immunoprecipitation (ChIP) assays in the Arabidopsis seeding expressing rSPL9-GFP or GFP only. The locations of SPL9 predicted binding sites (SPB-box, black boxes) were illustrated in the MAC3A and MAC3B promoters (upper panel). The amplicons were designed to surround SBP-Box (greys boxes labeled with A to H) or ACTIN2 ( ACT2 ) as controls. The chromatins in the Arabidopsis seedlings expressing rSPL9-GFP (dark blue bars) or GFP (grey bars) along with Col-0 were immunoprecipitated with anti-GFP antibody, and the relative enrichments of immunoprecipitated DNA relative to input were calculated as “%input.” shown as a bar graph. The fold enrichment (black dots) was calculated by dividing “%input” in SPL9-GFP with “%input” in GFP. The grey horizontal dashed line represents the fold enrichment of negative control, ACT2 . The “ND” stands for not detectable, and the transcription start site (TSS) and the translation initiation site (TIS) were labeled. Only one replicate is presented here.

    Journal: bioRxiv

    Article Title: MOS4-Associated Complex subunits 3A and 3B modulate FLM splicing to repress photoperiod-dependent floral transition

    doi: 10.1101/2024.03.26.586198

    Figure Lengend Snippet: (A) SPL9 interacts with the MAC3A and MAC3B promoters in the yeast one-hybrid assays. The yeast bearing the pLacZi promoter-reporter vectors and pGADT7 vectors expressing SPL9 were grown on synthetic dropout medium (SD-Leu-Ura-Met). The colonies were blotted on the filter paper to test the β-Galactosidase (β-Gal) activity. The blue color indicates the interaction between 1.5 kb promoter fragments of MAC3A and MAC3B in the pLacZi vectors and the transcription factors in the pGADT7 vectors. Empty vectors (e.v.) were used as negative control. (B) The chromatin immunoprecipitation (ChIP) assays in the Arabidopsis seeding expressing rSPL9-GFP or GFP only. The locations of SPL9 predicted binding sites (SPB-box, black boxes) were illustrated in the MAC3A and MAC3B promoters (upper panel). The amplicons were designed to surround SBP-Box (greys boxes labeled with A to H) or ACTIN2 ( ACT2 ) as controls. The chromatins in the Arabidopsis seedlings expressing rSPL9-GFP (dark blue bars) or GFP (grey bars) along with Col-0 were immunoprecipitated with anti-GFP antibody, and the relative enrichments of immunoprecipitated DNA relative to input were calculated as “%input.” shown as a bar graph. The fold enrichment (black dots) was calculated by dividing “%input” in SPL9-GFP with “%input” in GFP. The grey horizontal dashed line represents the fold enrichment of negative control, ACT2 . The “ND” stands for not detectable, and the transcription start site (TSS) and the translation initiation site (TIS) were labeled. Only one replicate is presented here.

    Article Snippet: The promoter regions of MAC3A (−1487 to +266) and MAC3B (−1487 to +266) were cloned into pLacZi-GW reporter vectors, which incorporate a Gateway cassette between the SmaI and XhoI sites of the original pLacZi vectors (Clontech).

    Techniques: Expressing, Activity Assay, Negative Control, Chromatin Immunoprecipitation, Binding Assay, Labeling, Immunoprecipitation